Help for the Redox Proteins Tutorial

Redox Proteins is an Internet tutorial intended as a computer-based laboratory exercise for undergraduate students in biochemistry. It was designed to accompany the textbook Biochemistry (2nd ed.), by C.K. Mathews and K.E. van Holde (Benjamin/Cummings, 1996), but can be used together with any advanced course on biochemistry and biophysics.

The following topics are covered:

1. Organic redox cofactors
1. Nucleotides
2. Quinones
2. Redox enzymes with organic cofactors
1. GAPDH - overall structure
2. GAPDH - details
3. Alcohol dehydrogenase
3. Cytochromes
1. B-type cytochromes
2. C-type cytochromes
3. Cytochrome f
4. Nitrite reductase (cytochrome cd₁)
4. Iron-sulfur proteins
1. Single Fe
2. Fe₂S₂
3. Fe₄S₄
5. Copper proteins
1. Plastocyanin
2. Other type 1 copper proteins
3. Type 2 copper proteins
4. Nitrite reductase
6. Complexes of redox proteins
1. Cytochrome c peroxidase/cytochrome c
2. Methylamine dehydrogenase/amicyanin/cytochrome c
3. Cytochrome f/plastocyanin
7. Multisubunit redox proteins
1. Photosynthetic reaction center (Rhodobacter sphaeroides)
2. Photosynthetic reaction center (Photosystem I)
3. Cytochrome c oxidase

The tutorial makes use of the Chemscape Chime plug-in and JavaScript 1.1. Thus, Netscape Navigator 3.01 is required. The Chemscape Chime plug-in from MDL Information Systems Inc. (http://www.mdli.com/) makes it possible to embed molecular graphics in the HTML pages. Complex biomolecules can be displayed at various levels of atomic detail and the user can rotate and magnify the molecules to experience their three-dimensional structure. Particular attention has been paid to the design of the user interface so that students which are not so experienced with computers can navigate easily through the tutorial. A form with simple questions is included. Extensive help is available to guide the user.

A link to the tutorial can be found at the following URL: http://www.bcbp.gu.se/ (select Courses from the menu).